Influence of the polymerization method on the properties of fibrin matrices

Highlights . We have proposed and patented a method for obtaining fully autologous fibrin without the use of exogenous thrombin, which can be implemented within framework personalized approach in tissue engineering. Such fibrin, addition to lower risk infection inflammation, possesses greater strength resistance degradation, as well better hemocompatibility compared polymerized with is an undoubted advantage when used coating small-diameter vascular grafts. Background Autologous impart biomimetic properties various polymers Traditionally, fibrinogen polymerization performed thrombin calcium chloride. Our allows us obtain completely material that does not infection. The differences methods change properties. Aim To compare most important engineering obtained by methods: using endogenous vitro Methods precipitate was ethanol precipitation low concentration ethanol. content normalized final 30 mg/mL. Polymerization adding 50 U/ mL 0.2% chloride precipitate, activation only samples were tested on Z tensile tester (Zwick/ Roell). structure studied scanning electron microscopy, quantitative analysis size pores fibers, density branching points carried out. FXIII plasma resulting determined ELISA. activity formation ?-? crosslinks SDS-PAGE, proteolytic fibrinolytic degradation. ability activate contact coagulation platelet aggregation evaluated. Results Thinner fibers predominated samples, while distinguished stiffness. described features may associated more efficient FXIII, confirmed larger number dimers degradation thrombin. Moreover, activated platelets less than Conclusion polymerizing makes it possible has physical mechanical properties, thrombogenicity traditional polymerization.